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Bio-Rad blocking solution
Blocking Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+cd163/Mouse+anti+Human+CD163/pm41937075-87-39-36
Average 94 stars, based on 237 article reviews

blocking solution - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Amino acid 138 in the HA of a H3N2 subtype influenza A virus increases affinity for the lower respiratory tract and alveolar macrophages in pigs
Article Snippet: Briefly, Fc cell surface receptors were blocked by incubating cells with PBS-EDTA containing 5% porcine serum (ThermoFisher Scientific, Waltham, MA) and 5% horse serum (ThermoFisher Scientific, Waltham, MA) for 30 minutes on ice. .. Afterward, cells were incubated for 30 minutes on ice with the following antibodies in a 1:200 dilution: Alexa 647-conjugated anti-pig SLA class II DR clone 2E9/13 (Bio-Rad, Hercules, CA), RPE-conjugated anti-pig Monocyte/Granulocyte clone 74-22-15 (Bio-Rad, Hercules, CA), and Alexa 488-conjugated anti-human CD163 clone EDHu-1 (Bio-Rad, Hercules, CA). .. For FLUAV detection in BALF samples, cells were incubated with the aforementioned antibodies in addition to an anti-Multi-Hemagglutinin (H3N2) polyclonal antibody in a 1:500 dilution followed by a 1-hour incubation with a PE-Alexa 610-conjugated anti-rabbit secondary antibody at a 1:500 dilution (ThermoFisher Scientific, Waltham, MA).

Article Title: Magnetic‐Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering
Article Snippet: Then incubated with secondary antibody Alexa Fluor 647 goat anti‐rabbit (Thermo Fisher Scientific) at a 1:300 ratio for 1 h at RT, protected from light. .. Samples were then washed and blocked again with BSA 1% in PBS for 1 h at RT followed by incubation with primary antibody mouse anti‐human CD163 (M2 marker) (Bio‐rad) at a 1:100 ratio at 4 °C and later incubated with secondary antibody Alexa Fluor 594 goat anti‐mouse (Thermo Fisher Scientific) at a 1:300 ratio for 1 h at RT. .. The samples were visualized by confocal laser scanning microscope (Leica SP5) using Leica Application Suite X (LAS X) software.

Immunohistochemical staining:

Article Title: Tumor-Associated Macrophages in Canine Oral and Cutaneous Melanomas and Melanocytomas: Phenotypic and Prognostic Assessment
Article Snippet: .. Before immunohistochemical analysis, mouse anti-human Iba1 (Merck Millipore, Burlington, MA, US), anti-human CD163 (clone EDHu-1; Bio-Rad; Hercules, CA, US), and anti-human MRS-A/CD204 (clone SRA-E5;Cosmo Bio Co., LTD; Yuseong-Gu, Daejeon, Republic of Korea) antibodies were validated for detection of the canine proteins by Western blotting (WB), as previously reported ( ). .. Forty mg of fresh tissues (lymph node harvested from a dog dead from trauma and placenta collected immediately after a spontaneous delivery) were homogenized in lysis buffer (Cell Signaling; Danvers, MA, US) containing protease inhibitor cocktail (Sigma-Aldrich) and centrifugated for 13,000 × g for 15 min at 4°C.

Article Title: Tumor-Associated Macrophages in Canine Oral and Cutaneous Melanomas and Melanocytomas: Phenotypic and Prognostic Assessment.
Article Snippet: .. Before immunohistochemical analysis, mouse anti-human Iba1 (Merck Millipore, Burlington, MA, US), anti-human CD163 (clone EDHu-1; Bio-Rad; Hercules, CA, US), and anti-human MRS-A/CD204 (clone SRA-E5;Cosmo Bio Co., LTD; YuseongGu, Daejeon, Republic of Korea) antibodies were validated for detection of the canine proteins by Western blotting (WB), as previously reported (43). .. Forty mg of fresh tissues (lymph node harvested from a dog dead from trauma and placenta collected immediately after a spontaneous delivery) were homogenized in lysis buffer (Cell Signaling; Danvers, MA, US) containing protease inhibitor cocktail (Sigma-Aldrich) and centrifugated for 13,000× g for 15min at 4◦C.

Western Blot:

other:

Article Title: Association of COVID-19 inflammation with activation of the C5a-C5aR1 axis
Article Snippet: DPBS (1X) (14190-094, Gibco); RPMI medium 1640 (1X) (31870-025, Gibco); Sodium pyruvate 100 mM (100X) (11360-039, Gibco); L-glutamine 200 mM (100X) (25030-024, Gibco); Minimum essential medium non-essential amino acids solution (11140-035, Gibco); Trypan blue stain (0.4%) (15250-061, Gibco); Ficoll-Paque PLUS (17-1440-03, GE Healthcare); Fetal bovine serum (F7524, Sigma); Dimethyl sulfoxide (D2650-100ML, Sigma); CD14 microbeads human (130-050-201, Miltenyi); EasySep direct human neutrophil isolation kit (19666, Stemcell); Bovine serum albumin (A9418-100G, Sigma); UltraPure 0.5 M EDTA, pH 8.0 (15575-038, Invitrogen); LPS EK ultrapure (tlrl-peklps, Invivogen); R848 (tlrl-r848, Invivogen); C5a (IPH 1D9 batch 1A, Innate Pharma); C5a (2037-C5-025, R&D Systems); Avdoralimab (Innate Pharma), Isotypic control (Fc-silent hIgG1) (Innate Pharma); CD33-PECF594 clone WM53 (562492, BD Biosciences); CD19-PECy7 clone SJ25C1(557835, BD Biosciences); CD3-BUV496 clone UCHT1 (564809/612940, BD Biosciences); CD15-BV510 clone W6D3 (563141, BD Biosciences); CD45-BV711 clone HI30 (564357, BD Biosciences); CD16-BUV395 clone 3G8 (563785, BD Biosciences); CD14-BUV737 clone M5E2 (564444/612763, BD Biosciences); HLA-DR-AF700 clone L243 (307626, BioLegend); LIVE DEAD NEAR IR (L34976, ThermoFisher); Mouse serum (015-000-120, Jackson ImmunoResearch); CD88 C5aR PE clone S5/1 (344304, BioLegend); Anti-CD16 FITC (556616, BD Biosciences); Anti-CD11b PE-Cy5 (555389, BD Biosciences); U-PLEX kit (N05235A-1, MSD); OptEIATM huC5a ELISA (557965, BD Biosciences); Mouse Albumin ELISA Kit (E99-134, Bethyl Laboratories); Ficoll (11778538, Invitrogen); Dextran (31382, Sigma); Calcein AM (C3100MP, Invitrogen); Fibrinogen (F3879, Sigma); Transwell Fluoroblok 3 μm insert (351151, Corning); EDTA (15575-038, Invitrogen); Sodium azide (71290-100g, Sigma); Optilyse C solution (A11895, Beckman Coulter); CytoFix (554655, BD Bioscience); Avacopan (HY-17627, Clinisciences); Anti-C5a (Innate Pharma); Anti-mouse Ly-6C BV510 clone HK1.4 (128033, BioLegend); Anti-mouse Ly-6G BV786 clone 1A8 (740953, BD Biosciences); Anti-mouse CD45 BUV395 clone 30F11 (564279, BD Biosciences), Anti-mouse CD11b BUV737 clone M1/70 (564443/612800, BD Biosciences); Anti-mouse C5aR1-APC clone 20/70 (130-106-124, Miltenyi Biotec); Anti-human CD88 clone S5/1 (HM2094-100UG, Hycult Biotech); Anti-human CD68 clone KP1 (M0814, Agilent); Anti-human CD163 clone EDHu-1 (MCA1853, BioRad).

Article Title: Protective effect of heat-inactivated Mycobacterium bovis applied intramuscularly is associated with enhanced lung immune response in caprine tuberculosis
Article Snippet: Anti-human CD163 , M2 MΦs , mAb , 1:200 , TC-autoclave b , Bio-Rad AbD Serotec.

Immunopeptidomics:

Article Title: Spatially resolved single-cell atlas unveils a distinct cellular signature of fatal lung COVID-19 in a Malawian population
Article Snippet: .. rabbit anti-SARS-CoV-2 nucleocapsid protein (5ug/ml) Novus Biologicals Cat# NB100-56576 rabbit anti-human CD31 (2ug/ml) Novus Biologicals Cat# NB100-2284 rabbit anti-human SP-C (4.17ug/ml) Novus Biologicals Cat# NBP1-60117 mouse anti-human CD68 (5ug/ml) Agilent Cat# M0876 rabbit anti-human KRT8 (2.5ug/ml) Abcam Cat# ab59400 anti-human Smooth muscle actin (SMA) (clone 1A4) Bio-Rad Cat# MCA5781GA anti-humanCD68 (clone KP1) Thermo Cat# MA5-13324 anti-human CD235ab (clone HIR2) BioLegend Cat# 306615 anti-human Pan-cytokeratin (PanCK) (clone AE-1/AE-3) Biolegend Cat# 914204 anti-human CD38 (clone EPR4106) Standard Biotools Cat# 3141018D anti-human MHC I (clone EMR8-5) Abcam Cat# ab70328 anti-human Vimentin (clone RV202) Standard Biotools Cat# 3143029D anti-human CD14 (clone D7A2T) Cell Signalling Cat# 56082BF anti-human ICAM1 (clone EP1442Y) Abcam Cat# ab271852 anti-human CD16 (clone SP175) Abcam Cat# ab243925100ug anti-human iNOS (clone SP126) abcam Cat# ab239990 anti-human CD66b (G10F5) Novus Cat# g10f5_nb10077808 anti-human CD11b (EP1345Y) Abcam Cat# ab5247 anti-human CD44 (IM7) BioLegend Cat# 103001 anti-human CD107a (H4A3) Standard Biotools Cat# 3151021D anti-human CD45 (CD45-2B11) eBioscience Cat# 14-9457-82 anti-human CD31 (JC/70A) Novus Cat# jc-70a_nb600562 anti-human CD11c (Polyclonal) Standard Biotools Cat# 3154025D anti-human Foxp3 (clone 236A/E7) Abcam Cat# ab20034 anti-human CD4 (clone EPR6855) Standard Biotools Cat# 3156033D anti-human SARS-Cov-2 (Polyclonal) Novus Cat# NB100-56576 anti-human Von Willebrand Factor (vWF) (Polyclonal) Dako Cat# A0082 anti-human Vista (clone D1L2G) Standard Biotools Cat# 3160025D anti-human CD20 (clone H1) Standard Biotools Cat# 3161029D anti-human CD8 (clone CD8/144B) eBioscience Cat# 14-0085-82 anti-human Iba1 (Polyclonal) WAKO Cat# 019-19741 anti-human Arginase 1 (clone D4E3M) Standard Biotools Cat# 3164027D anti-human Fibrinogen (EPR18145-84) Abcam Cat# ab227063 anti-human CD74 (clone LN2) Standard Biotools Cat# 3166025D anti-human Granzyme B (clone EPR20129-217) Standard Biotools Cat# 3167021D anti-human Collagen Type I (Polyclonal) Standard Biotools Cat# 3169023D anti-human CD3 (clone D7A6E) Cell Signalling Cat# 85061BF anti-human pERK1/2 [T202/Y204] (clone D13.14.4E) Standard Biotools Cat# 3171021D anti-human Cleaved Caspase 3 (clone 5A1E) Standard Biotools Cat# 3172023A anti-human CD45RO (clone UCHL1) Standard Biotools Cat# 3173016D anti-human MHC II (clone TAL1B5) Abcam Cat# ab176408 anti-human CD206 (clone E2L9N) Cell Signalling Cat# 91992 anti-human CD163 (clone EDHu-1) Bio-Rad Cat# MCA1853 .. Package name Reference (where applicable) URL R v4.3.0 https://www.cran.r-project.org Python v3.9.6 https://www.python.org RStudio software (v 2023.03.0+386; 2023) The R project for Statistical Computing https://www.rstudio.com/ and https://cran.r-project.org/ Ilastik v1.4.0 Berg et al., 2019 https://www.ilastik.org CellProfiler v4.2.4 https://cellprofiler.org FactoMineR v2.8 (R package) Sebastien Le, 2008 https://cran.r- project.org/web/packages/Fact oMineR/index.html ggplot2 v3.4.2 (R package) https://ggplot2.tidyverse.org factoextra v1.0.7 (R package) https://cran.r- project.org/web/packages/facto extra/index.html corrplot v0.92 (R package) https://cran.r- project.org/web/packages/corr plot/vignettes/corrplot- intro.html missMDA v1.18 (R package) Josse and Husson, 2016 https://cran.r- project.org/web/packages/miss MDA/index.html tidymodels v1.0.0 (R package) https://www.tidymodels.org cytomapper v1.9.2 (R package) Nils Eling, 2020 https://github.com/Bodenmiller Group/cytomapper miloR v1.4.0 (R package) (Dann et al., 2022) https://marionilab.github.io/mil oR Giotto v3.3.0 (R package) Dries et al., 2021 https://giottosuite.readthedocs. io/en/latest/ ImcSegmentationPipeline (Python package) Vito RT Zanotelli, 2022 https://github.com/Bodenmiller Group/ImcSegmentationPipelin e IMC-Denoise (Python package) Lu et al., 2023 https://github.com/PENGLU- WashU/IMC_Denoise DeepCell (Python package) Greenwald et al., 2022 https://github.com/vanvalenlab /deepcell-tf Scanpy (Single-Cell Analysis in Python) v1.9.1 (Python package) (Wolf et al., 2018 https://github.com/scverse/sca npy umap-learn v0.5.3 (Python package) (McInnes, 2018 https://github.com/lmcinnes/u map Astir (ASsignmenT of sIngle-cell pRoteomics) v0.1.4 (Python package) Geuenich et al., 2021 https://github.com/camlab- bioml/astir Squidpy (Spatial Quantification of Molecular Data in Python) v1.2.2 (Python package) Palla et al., 2022 https://squidpy.readthedocs.io/ en/stable/ Athena (Analysis of Tumor HEterogeNeity from spAtial omics measurements) v 0.1.3 (Python package) (Martinelli and Rapsomaniki, 2022 https://ai4scr.github.io/ATHENA /index.html SpOOx (Spatial Omics Oxford Pipeline) (Python package) (Praveen Weeratunga, 2022 https://github.com/Taylor-CCB- Group/SpOOx Supplemental Figure 1 | Histological characteristics of lung pathology in patients with SARS CoV-2 infection A B C D E F * * * * * * * * * * * * ** * * * * Supplemental data, Fig. 1 Supplemental Figure 2 | Top cluster markers characterising immune and stromal cell populations in the lung in the Malawi cohort Supplemental Figure 3 | Characterisation of cell populations in the nasal and peripheral blood tissue compartments in the Malawi cohort.

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blocking solution (Bio-Rad)

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Western Blot:

other:

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